Summary

A combination of Surface Enhanced Raman Spectroscopy (SERS) and Light Sheet Microscopy (LSM) is reported. We combine Surface Enhanced Raman Spectroscopy and Light Sheet Microscopy because SERS corrects the intrinsically low Raman Signal of molecules and LSM decreases photo toxicity and increases the speed in image and spectral acquisition. Therefore, this non-invasive novel technique would allow studying dynamic processes of living organisms. Furthermore, bigger specimens such as tissues and full organisms could be studied taking advantage of LSM configuration. As proof of concept, we will use pH nanosensors. They consist in Hollow Capsules covered with a thin silica shell. Inside, we find Gold nanoparticles to produce SERS effect and the pH sensor, a molecule with strong pH sensitive and insensitive characteristic peaks in the Raman Spectrum used for tracking the pH.